Pharmacodynamic differences between hangmaidong and chuanmaidong based on metabonomics / 药学学报

To evaluate the differences of Ophiopogonjaponicus from different cultivations, the metabolomics based method was conducted to compare the effects of Hangmaidong and Chuanmaidong (Chinese name) on plasma endogenous metabolites of normal rats. Data were collected by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS), and were analyzed by multivariate statistical method, such as Principal Component Analysis and Orthogonal Signal Correction Partial Least Square Discriminant Analysis. Results revealed that the plasma metabolites profiling of low and middle dose group of Chuanmaidong were similar to the control group, but different from the high dose group obviously. Meanwhile the high, middle and low dose groups of Hangmaidong were different from control group notably, and the difference is dose dependent. Lysophosphatidylcholines, the possible endogenous metabolites which contribute to the classification most significantly, are closely related to cardiovascular system diseases. Compared with the group of Chuanmaidong, Hangmaidong has greater impact on the plasma metabolic profiling of normal rats. Hangmaidong and Chuanmaidong showed significant differences pharmacodynamically.

Soil microbial community composition and diversity in Panax quinquefolius rhizosphere / 中国中药杂志

The objective of this work was to analyze the soil microbial community diversity and structure in Panax quinquefolius rhizosphere and elucidated the rules of the ecology shift. Two community-based microbiological measurements, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid ester-linked fatty acid (PLFA) profiles, were used to evaluate soil microbial community function and composition of different Panax quinquefolius cropping soils. The properties, pH, N, P, K and organic matter were analyzed also. There were many differences in soil properties such as lower pH (5.82) in Jilin soil but higher pH (8.27) in Beijing soil and there were significant higher content of organic matter, available N, P, K in Jilin soil than those in Beijing soil. The analysis of soil microbial ecology showed a similar result in AWCD, Biolog and PLFA pattern. According to the PC1 score in principal analysis of Biolog and PLFA, the soils in 2, 3 and 4 years cultivated P. quinquefolius rhizophere were distinguished from the control and 1 year soils. The result indicated that the metabolism function reduced and the structure shifted in rhizophere soil microbial community after P. quinquefolius cropping. These may be the primary reason of the disease occur severely in P. quinquefolius field.

Effects of seeds irradiation with 60Cogamma-ray on shoot growth and physiological status of Carthamus tinctorius / 中国中药杂志

<p><b>OBJECTIVE</b>To provide the radiation-induced technical reference and theoretical basis for safflower and other medicinal plants.</p><p><b>METHOD</b>Seeds of Carthamus tinctorius were irradiated with 60Cogamma-ray, and germination rate of seeds, germination, seedling rate and seedling height, root length, fresh weight, root activity and peroxide catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) activity were determined.</p><p><b>RESULT AND CONCLUSION</b>The LD50 of radiation dose is about 300 Gy, effects of seeds irradiation with 6Co-gamma-ray on shoot growth and physiological status of C. tinctorius are obtained.</p>

Multiplication of virus-free seedlings of Rehmannia glutinosa CV. 85-5 in vitro / 中草药

Object To develop a method of multiplying virus free clonal seedlings of CV.85 5 of Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey.. Methods The leaf cut segments with or without tips of plantlets were grown in different media (mg/L) (1.MS+BA 0.5; 2 MS+BA 0.5+NAA 0.02;3 MS+BA 0.5+NAA 0.02+GA 3 0.1; 4 1/2 MS+BA 0.1; 5 1/2 MS+BA 0.1+GA 3 0.1; 6.1/2 MS+GA 3 0.1) to select a suitable medium, and the height and the numbers of newly formed leaves and roots were recorded 30 days later. To screen out a favorable condition, similar segments were transferred to No.2 medium at different temperatures in different illuminations, and the height together with the leaf number and the fresh weight of the plantlets was recorded.Results In the former three media, the segments with tips were flourishing and one to three axillary buds were formed at its basal stem; while in those without tips almost every axillary bud developed with the main tip length 0.5～1.5 cm. In the later three media, thd elongation of the segments was usually overstimulated, and the slim plantlets with fewer leaves and more roots formed. When the segments cultured in No.2 medium, all records at 28 ℃ were higher than those at 23 ℃ in the same illuminations. But the leaf size and the height of the plantlets were inversely proportional to the intensity of illumination at the same temperature. In addition, antagonism between BA and GA 3 was found.Conclusion No.2 medium is more suitable for the multiplication of the virus free seedlings of 85 5 when cultured at 28 ℃ in the illumination of 1 000～2 000 lx.

Sources of sugar in flowers and fruits of Macrocarpium officinale during fruiting / 中草药

Object To study the source of sugar in flowers and fruits of Macrocarpium officinalis (Sieb et Zucc.) Nakai during fruiting.Methods The sugar contents were analyzed by HPLC.Results The contents of glucose, fructose and total sugar decreased slowly in the third or the forth branch after the barks were cyclically cut and there were significant differences between test group and control group, the changes of sugar contents in the first or the second branch were similar to control group.Conclusion These sugars in flowers and fruits were provided by the first or the second branch during fruiting.

Physiological and Biochemical Changes During Embryo After-Ripening of American Ginseng(Panax quinquefolius)and Effect of Hormones on Such Changes / 中草药

In natural condition, two years were needed for Panax quinquefolius seeds to break dormancy. In the first year, the embryo developed slowly, and the activities of amylase and peroxidase were low. In the second year the embryo developed rapidly, and the activities of both enzymes also increased gradually. But with the treatment of hormones enzymatic activ ities accelarated remarkably and speeded up the decomposition of stored nutrients in endos perm, so that starch and non-reducing sugar decreased while reducing sugar incrcased rapidly, resulting in earlier germination.

Regulation of dry substance and active components accumulation in fruit of Forsythia suspensa / 中草药

Objective To study the regulation of dry substance and active components accumulation in the fruit of Forsythia suspensa. Methods The dry substance of the fruit was analyzed by the method of hundred grain mass. The contents of the active components in leaves, shucks, and seeds were analyzed by HPLC. Results The period for F. suspensa to accumulate the dry substance was mainly May and July. During the whole growth phase, the active components, such as forsythiaside, forsythin, and rutin in different parts, were decreasing. From the last ten-day of August to the first ten-day of September, the accumulation of the dry substance came to stability and the contents of active components in shucks and seed were stable. The contents of active components in shucks decreased rapidly from September to October, and then came to stability and the contents in shucks is the least during the last ten-day of October. So the contents of active components in younger F. suspensa are higher than that in elder one, and the proportion among forsythiaside, forsythins, and lution is also significantly different from elder seeds. Conclusion It is more preferable to pick younger F. suspensa from the last ten-day of August to the first ten-day of September and to pick elder F. suspensa during last ten-day of October.